Compose a 1250 words essay on History of analytical techniques used in structure determination. Needs to be plagiarism free!
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The map tends to be fuzzy in some parts this is due to the problem of phasing loops but with the help of some software can usually predict up to 90% of the structure correctly and the remaining part is computed manually. The problem in this method is that it is very expensive and takes time and we can determine structure of only large crystal.
The structural determination of protein is difficult by method because Crystallography requires purified protein in fairly large amount and it requires protein in form of a crystal and every protein can’t be crystallized. This is due to the fact that forming the crystal required rather unusual conditions of pH and ionic strength.
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